![]() ![]() If your program requires many DLLs I think is better to just distribute them as external files along with your program. I do not wish to use the Packer commands. For example For a small tech-demo, intro, utility, etc. However, I cannot find a PureBasic command, or cross-platform (I can only find Windows solutions I am coding on OS X.) way of getting the image memory data to save with this command. The idea is to embed just 1 or 2 DLLs in your program since sometimes may be annoying to have to distribute them separately, Load all the DLLs you want from memory as long their dependencies are available in the file system (tipically OS DLLs : GDI32, KERNE元2, MSVCRT. Indeed you have, but that is a PureBasic-managed object, and therefore does not maintain a fixed memory location. Load all the DLLs you want from memory as long they are not dependent on each other. It's not possible to load from memory a DLL "A" depending on a DLL "B" if "B" is also embedded in memory and not available in the filesystem. 8) The #DLL_PROCESS_ATTACH event is signaled to the DLL and its handle is returned. 7) The protection flags specified in the section headers are applied to the sections in memory. 6) If the DLL has not been mapped to the preferred location, the relocation table is processed and relocations are applied. With over 600 projects, there is hopefully one that you will find interesting and valuable to your development. 5) If any of functions is forwarded to another DLL, the target DLL module is retrieved if the DLL is already in memory, else the required DLL is loaded. Note: Direct memory manipulation must be handled with care. This library allows to allocate any number of memory buffers and to use them directly within PureBasic. 4) Process the import table, load the required DLLs (dependencies) and patch the table with the loaded function addresses. Sometimes it's very useful have raw access to the system memory (RAM) to process some time consuming operations and speed them up. The user has the choice to statically allocate buffers, allocate them on the stack, or allocate on the heap furthermore, it is possible to avoid memory fragmentation. 3) Map the DLL to virtual space, copies the headers and the sections in memory to the appropriate aligned starting offsets. The complete lack of heap allocation makes this code suitable for constrained environments such as operating system kernels and small microcontrollers (e.g. 2) Reserve enough virtual address space to map the DLL to it, at the preferred address specified in the DLL if possible, else at a different one. 1) Check if the image in memory looks like a valid DLL. The 圆4dbg debugger () to enable me to track a problem. Countless Microsoft articles and text files describing the PE format. ![]() The user "shebaw" ( forum) for some nice C snippets I converted to PB. Joachim Bauch () for the original code I studied to learn something about what a loader should do. Feel free to report if a DLL doesn't work for you and I'll try to improve the code if I can. Tested on Windows 7 and Windows 10 with just *ONE* DLL both in 32 and 64 bit flavours. Reading text from any image using Microsoft Office 2007 OCR. With this module is possible to load a DLL from memory instead of a file. FingerPrintf: A Small Library for Quick Usage of the Biometric API, Fingerprint and Camera. Fixed a bug: SizeOfRawData is not VirtualSize ! OS: Tested on Windows 7, Windows 10, x86 and 圆4 with PB 6.00 This includes all allocations and freeing of resources. I hope you are not suggesting that PB should allocate 64k for all strings by default just in case you want to use one with a certain API command?Ī far more sensible approach is that because you the programmer know what you want to do, then you should do it. ![]() Why does PB have to allocate the maximum length of string (the maximum is currently around 64k)? How does it know you want to use a string for the buffer of an API command? Parameters for API commands are only tagged as being addresses, not strings or buffers so the compiler cannot decide what needs to have strings allocated before they are used. The repository provides code for running inference with the SegmentAnything Model (SAM), links for downloading the trained model checkpoints, and example notebooks that show how to use the model. If yes, then PB has to put it with the max lenght. The purifier can detect a certain type of programming errors such as writing past the end of an allocated memory. I don't remember if len of string in PB are limited ? This enables purifier support for the debugger. ![]()
0 Comments
![]() Orbital Sidekick launched the first two satellites of its six-satellite Global Hyperspectral Observation Satellite, or GHOSt, constellation to provide hyperspectral imagery. Two others were from its space services business: one for Saudi Arabia’s King Abdullah University of Science and Technology equipped with a hyperspectral camera and a sensor for measuring soil moisture, while the ADLER-2 satellite for the Austrian Space Forum and Findus Venture GmbH will characterize the debris environment of low Earth orbit and study atmospheric aerosols. Spire had three satellites on the launch, but only one was for its own network for collecting weather, maritime and aviation tracking data. ![]() Umbra launched another synthetic aperture radar imaging satellite. HawkEye 360 launched its seventh cluster of three satellites for radiofrequency (RF) monitoring and Unseenlabs launched its BRO-9 satellite, also for RF monitoring. Satellogic had four imaging satellites onboard while GHGSat had three satellites for greenhouse gas monitoring. Several companies used the launch to augment existing constellations of satellites. This was the first in SpaceX’s series of Transporter dedicated smallsat rideshare missions to launch from Vandenberg, after the first six launched from Cape Canaveral, Florida. The launch carried 51 payloads, SpaceX said, although it did not release a full list of the satellites on board. On this flight, though, the booster reversed that, firing a single engine for the entry burn but three for landing, an approach SpaceX has previously used for landings of Falcon Heavy side boosters but not for the Falcon 9. Falcon 9 missions have previously used three engines on its entry burn and a single engine for landing. That affected the landing of the booster. Company representatives said on the launch webcast that they will use the shorter nozzle, which saves money, on launches that do not need as much performance, but retain the longer nozzle for higher-performance missions. It was the first Falcon 9 launch to use a shorter nozzle on the rocket’s upper-stage engine. This launch, the 23 rd Falcon 9 mission of the year, did have some novelties. ![]() The upper stage then performed two more maneuvers before deploying the final, and largest, payload, the 800-kilogram IMECE imaging satellite built by Turkish research institute Tübitak Uzay 2 hours and 35 minutes after liftoff. Those payloads were released over a span of about 20 minutes. Most of the payloads were deployed starting an hour after liftoff, following a second burn of the upper stage. The upper stage reached orbit a little more than eight minutes later, shortly after the first stage landed back at the launch site. Eastern, after several days of weather-related delays. The Falcon 9 lifted off from Vandenberg Space Force Base in California on the Transporter-7 mission at 2:48 a.m. Murloc Mondays (New Players Q&A) Reset Recruitment Midweek Mending Tanking Thursday Firepower Friday (DPS) Saturday Achievement and Loot Thread Skirmish Sundays (PvP) Blizzard Subredditsĭiablo - Starcraft - Hearthstone - Heroes of the Storm - Overwatch - WC3 - Classic WoW - Mystery -įrequently Asked Questions about Dragonflightįrequently Asked Questions.WASHINGTON - SpaceX launched more than 50 satellites early April 15 on the latest in a series of dedicated Falcon 9 smallsat rideshare missions. Undermine Exchange - useful resource for goldmakers. ![]() /r/classicwowtbc - World of Warcraft: Burning Crusade./r/classicwow - World of Warcraft: Classic./r/transmogrification - show us your style./r/woweconomy - goldmaking discussion & theory./r/wownoob - if you are a noob or want to help a noob.WoW Related Discords Dragonflight Patch 10.1.5 is Live! If you would like to be a future featured creator, please fill out this form. More information about our current & past featured creators can be found here. Welcome to /r/wow, a subreddit about the video game World of Warcraft ! Featured Creators ![]() ![]() ![]() In the case of Shuten Dōji, the titular drunken oni and his comrades can be seen as representative of voices outside of Japan’s imperial rule - making their somewhat sympathetic portrayal that much more compelling. “When I was teaching, I wanted to introduce Shuten Dōji because it’s such a famous story and it wasn’t in translation.”įor Reider, part of the oni’s allure is their deep symbolic meaning, often standing in for marginalized people or groups in society. “Shuten Dōji is the story that inspired me to start working on oni,” says Reider. Eventually, Shuten Dōji is defeated by a warrior-hero named Minamoto no Yorimitsu, who assaults the drunken demon’s palace and cuts off his head.Ī woodblock print by Japanese artist Utagawai Yoshitsuya (1822 - 1866) shows hero Minamoto slaying the ogre-like oni Shuten Dōji at his mountainous lair. One of Japan’s most well-known oni legends is the tale of Shuten Dōji, or “the drunken demon.” A powerful, fearsome oni, Shuten Dōji and his band of thugs rampaged through medieval Kyoto, capturing daughters of nobility to drink their blood and devour their flesh. They’re also known to emerge from the abyss of hell itself - or Jigoku in Japanese Buddhism - to frighten mortals, or torment evildoers who are sent there. In folklore, oni are often characterized as malicious troublemakers, capable of terrible deeds like spreading disease and attacking humans. But that, of course, is incorporated into Japanese oni, too." Legend of the Drunken Demon It’s like a zombie - those who are dead become oni. In Chinese, that character means something a little different. “And so we borrowed the writing system from China. “We didn’t have any writing system ,” adds Reider. Read More: Are Real-Life Vampires Walking Among Us? “And because of Chinese influences, the character for oni was taken from China.” “In every society, there is some evil, dark And Japanese did have evil deities and violent deities,” says Reider. As such, some scholars believe that the idea of the oni is purely Buddhist, while others claim that they aren’t exclusive to a particular theology. ![]() ) Other scholars, like Wakamori Tarō, argue that they came from inhabitants of ancient Japan who thought that they were evil spirits from deep in the mountains.Īnother theory suggests that the concept of oni (or at least the word itself) is Chinese in origin - and may have been introduced to Japan from China alongside Buddhism. In the Japanese line, for example, the oni’s genesis is attributed to people’s fear of the destructive power of natural forces like lightning, thunder and earthquakes, according to Kondō Yoshihiro, author of Nihon no oni: nihon bunka tankyū no shikaku (or Japanese Oni: Perspectives on the Search for Japanese Culture. Indeed, in her book Japanese Demon Lore, Reider describes four distinct cultures and traditions where oni stories stem from: Japanese, Chinese, Buddhist and onmyōdō, a traditional Japanese blend of cosmology, occultism and natural sciences based on the Chinese theories of “yin and yang.” Given their amorphous, ever-changing nature, it’s perhaps no surprise that onis have no single, unifying origin story. “Before, an oni could identify as male, female or ‘it.’ An artificial object could even be an oni.” The Origins of Oni “When an oni is female, they are called kijo, which means ‘oni woman,’” adds Reider. (In some traditions and depictions, for example, they can bring prosperity to other humans.) And while their visual imagery is usually masculine - loincloth and all - there are plenty of female oni, some of whom are enchantresses born out of feelings of jealousy, vengeance and shame. Still, looking at oni in a different context shows that they aren’t always monstrous ogres with a taste for flesh. “That is the folkloric way that they’re often depicted in pictures.” “When we think about visualizations of oni, horns, skin color that’s red, green, black or blue with conspicuous canine teeth and masculine body types - with tiger-skin loincloths,” says Reider. ![]() (Credit: Public Domain/Columbia University) This detail of a Japanese print by famed artist Katsushika Hokusai (1760–1849) shows two oni - one with one eye and one with three eyes. ![]() ![]() The National Farmers Union of Scotland (NFUS) said lynx were blamed for a fifth of those losses. The scheme faces heavy opposition from Scottish farmers, who cite the loss of about 20,000 sheep in Norway to its native predators, the wolverine, lynx, bear and wolf. A prerequisite for any discussion over lynx is we need to know the Scottish public are in support of this and would tolerate this.” ![]() “The question with a large predator is less about science and more about people’s attitudes, beliefs and priorities. There have been several studies to assess the ecological feasibility of bringing back lynx that’s the easy bit in many ways. Pete Cairns, the director of Scotland: The Big Picture, said: “Our role is to get the process moving, to pick up the baton in a meaningful way. They have some great lectures too.The lynx, Europe’s largest native cat, became extinct in northern Britain more than 500 years ago through habitat loss, hunting and persecution, but proposals by other rewilding advocates to reintroduce the species into the UK have foundered.Ĭonservationists believe the new project, run by the Vincent Wildlife Trust and Trees for Life, alongside campaigners at Scotland: The Big Picture, has a significant chance of success. The Highland center is awesome - □ Go there and find your path to outdoor enjoyment and learning. We had the pleasure of purchasing a painting which then allowed us to meet the artist and attend an event at her house - it has introduced us to some lovely new friends and all due to the Highland Center. We look forward to continuing to enjoy, explore and support Highland nature center - many activities for young'uns and young'uns at heart. We chose to also purchase the "meal" and it was yummy and delightfully packaged - from Los Gatos. Highland Center coordinator and crew set up seats/tables around the discovery garden and it was enchanting as we enjoyed the pre-play time in that lovely atmosphere. We are addicted to going there.īut last night was the best - oh joy!!! A fairy tale setting for the play Merry Wives of Winslow - Shakespeare with a western twist!! The acting was excellent - wonderful dialogue and sarcasm - and the environment was absolutely superb. We have always loved the Highlands Center - a hidden treasure - with its lovely discovery garden and Pebble Loop Trail and all the trails - all are easy enough for most hikers. Trails are open til 6:00 in the winter and 7:00 in the summer, but be advised the store closes at 3:00 in winter and 4:00 in summer and is closed on Sunday and Monday. The center is funded by memberships and donations, so be generous if you visit the facility. We were told that they are constructing a "discovery garden" so that will be an added bonus! I would have liked to see more educational exhibits I was expecting a museum or nature center experience. There was also a restroom at the half-way point on the trail. The trail was hard-packed dirt and rocks with a few grades, so some assistance may be required. The area around the building and the 3/4 mile interpretive "Stretch Pebble Nature Trail" through the forest were wheelchair accessible. The ladies operating the nature store were very pleasant and helpful. The James Learning Center building and adjacent restrooms were clean and attractive. We stopped by to see the Highlands Center in April when we were visiting Lynx Lake Recreation Area, which is also on Walker Rd. There is no food or water for sale at the gift shop so bring your own and be sure to leave nothing behind but footprints. But after you've been there and done one of the trails you'll likely feel that some contribution is worth it to make this experience available to future visitors. Yes, there is a donation box if you're so inclined, but the objective of the Highlands Center is to expose as many people as possible to the wonders of nature rather than generate income. The longer trail focuses on the geologic history of the Prescott area, pointing out evidence of plate tectonics and an undersea rock genesis.Īnd the best part is that it's free. The emphasis on the shorter trail is ecosystems: how plants, animals, fungus, bacteria, insects and rocks interact in cycles and webs. Trail maps at a kiosk near the parking lot will lead you from one interpretive spot to the next. One can be done in as little as half an hour. ![]() The Highlands Center is a wonderful place to spend a couple hours or half a day. ![]() ![]() ![]() The common ground in current guidelines is that there are pointers to the lack of safety data on excipients and that available data generally does not apply to neonates requiring further justification including provision of non-clinical safety data. This is insufficient considering the much wider and complex needs of neonates and lack of clarity about how this limited guidance is to be translated into patient-centric product development, which meets with regulatory approval. The capability to administer small volumes in relation to dosing error is also mentioned. In these guidelines, a rather general and brief set of considerations are made in terms of formulation and around polypharmacy via parenteral routes of administration in the hospital setting. In terms of international guidance, it is only in the latest revision of the ICH E11 Guideline on Clinical Investigation of Medicinal Products in the Paediatric Population (2018) that neonates are specifically mentioned as an age classification and paediatric subgroup. The situation is not helped by a relative lack of relevant guidelines on the development of medicines for neonates (let alone those born prematurely). An international consortium of experts has produced a white paper to facilitate successful neonatal clinical trials of medicines and includes useful information on neonatal dosage forms and formulations. There is a ‘Catch 22′ situation because to protect neonates, trials have been deferred so that safety and efficacy data are obtained in older age groups meanwhile the unmet needs gap widens, necessitating off-label use. In fact, trials open for recruiting neonates were included in only a quarter of all agreed paediatric investigation plans (PIPs), often at the request of the EMA Paediatric Committee (PDCO), due to an array of reasons: Lack of neonate specific indications, recruitment/enrollment challenges, lack of incentives. Considering the extra challenges they present, pharmaceutical companies are not incentivised to formulate medicines for the neonatal population. This remains an even more significant problem in the neonatal population, due to the difficulty in conducting the necessary clinical trials in vulnerable subsets with lower patient numbers. In paediatric patients, there is still significant off-label use of medicines due to a lack of medicines developed and authorised for the specific needs of the very young. The recent European Medicines Agency (EMA) report to the European Commission on the experience acquired as a result of the first 10 years application of the Paediatric Regulation, acknowledged that neonates still represent a particularly neglected paediatric subpopulation in the development of medicines despite the regulatory push. This includes (amongst others) formulation aspects, dosage form choice, drug administration considerations and storage and handling advice. In turn, this can lead to potential lack of efficacy or reduced safety of a medicinal product leading to additional special requirements for development of products for these age groups. The underlying complexity of pharmacology and biopharmaceutics in neonates, especially in preterm babies, can lead to altered and variable pharmacokinetics and pharmacodynamics even compared to that in young babies. A host of other physiological factors such as gastro intestinal (GI) pH, body surface to volume ratio, body fat to lean tissue ratio are also different and are known to change rapidly with time. Additionally, the skin barrier may not be fully formed, and respiratory function may be immature. For example, neonates have reduced gastric emptying, intestinal transit time and surface area, and transporter immaturity, which have relevance for oral drug delivery. This immaturity of organ and function is more profound and impactful in preterm infants. Even if born at term and ready to grow outside of the mother’s womb, most organs and their functions are still immature. Įach year, some 15 million babies arrive in the world, more than one in 10 babies are born prematurely, according to the report ‘Born Too Soon: The Global Action Report on Preterm Birth’ (2012). The neonatal period for preterm newborn infants is defined as the day of birth through to the expected date of delivery plus 27 days. ![]() The neonatal period for term and post-term newborn infants is defined as the day of birth plus 27 days. ![]() Neonates include term, post-term and preterm babies. Neonates are not small adults, and neither can they be classified as small children when it comes to medicinal products and their formulation development. ![]() ![]() ![]() Prior to the removal of PsyScope 1.1.1, you should first log into your Mac with an administrator account, and turn PsyScope 1.1.1 off if it is running. To deactivate an app, right click its icon in the dock and select Quit option (or choose PsyScope 1.1.1 > Quit PsyScope 1.1.1 on the Menu bar).Īlso, you can access the Activity Monitor (located in /Applications/Utilities/ folder), and see if there is any background process pertaining to the app. Option 1: Drag PsyScope 1.1.1 icon to the Trash If PsyScope 1.1.1 cannot be quit normally, you can select the app in Activity Monitor, click the large red “Quit Process” button in the left corner and click Force Quit button in the pop-up dialog. ![]()
![]() ![]() At any rate, if she does decide to go, another soap will scoop her up quickly. ![]() ABC seemed to underestimate the power of her massive fan base, and may not be prepared for the backlash they could receive if Herbst leaves the show. Rebecca Herbst has a bright future, whether she stays on General Hospital or not. Their love is written all over their faces #GH #Liason #KeepBeckyGH /PRY6mZGqxv There are multiple possibilities for her character–if only the network is willing to work with her and meet her needs. For example, she could be the love interest for Dr. Becky has so much potential in the series, even without a Jason Morgan storyline. General Hospital fans are still hoping that Rebecca and the show can come to an agreement that would allow her to stick around another year. It is looking more possible that Rebecca Herbst could be the next person to leave the show. Jason moved to The Young and the Restless to portray Billy Abbott. Last year, General Hospital lost Anthony Geary after his contract talks went South, and just recently, Jason Thompson left the show after being disappointed in the way Dr. General Hospital has a habit of letting great talent slip through their fingers and seem unable to haggle with them to negotiate better contract terms. How can the fans accept that Liz would leave her kids in the hands of someone that she only recently decided to drop the hatch and get along? It hardly makes sense and seems like a terrible way to end Rebecca Herbst’s run as Elizabeth Webber. The ending for Nurse Webber bothered many of fans, because no matter how you feel about Liz, she tries her hardest to be a good mother. The rumor was that Liz Webber would leave her children with Sam (Kelly Monaco) and Jason (Billy Miller) Morgan to raise. Soap Central posted that if Rebecca Herbst decided to leave General Hospital, many fans would not want to see a recast, at least not for a while. ![]() His comments left fans scratching their heads– it wasn’t clear if he was the one who sent the private messages or if he was upset about being outed as talking about actor’s contract with fans, which is a violation of ABC current policy. In the end, Varni claimed that his Facebook was compromised however, he would no longer respond to private messages since his messages were publically released. Typical of Herbst, she showed that she is a true professional by not responding this week when ABC executive producer, Nathan Varni Facebook private messages were posted to groups, showing that he placed all the blame on her contract dispute on her. Rebecca has remained silent on if she plans to leave the ABC soap opera, or stay for another year. Would losing Rebecca Herbst be a loss to GH? #GH #TwoScoops /PJdIEQjSIa For some time, the GH fans have voiced their opinion on social media that the show is shelving many veterans to showcase the younger characters. General Hospital fans have been in a frenzy after Daytime Confidential hinted at Rebecca’s rumored exit. Petition Buzz revealed on Friday that Rebecca’s fans launched a campaign to save Herbst alter ego, Elizabeth Webber from leaving the show. There is no word what the accomplished daytime actress is looking for in her newest contract. It looks like her contract talks have hit a major snag, and it is probable that Becky could choose to walk away if the show doesn’t meet her demands. General Hospital (GH) spoilers tease that Rebecca Herbst may not be sticking around the ABC soap opera for long. ![]() ![]() ![]() ![]() For the non-root option, download and install MicroG from inside Vanced Manager.Download and install the latest version of Vanced Manager from the link provided on your Android device.To download the application, you need to follow the following steps: Version Info Nameĭownload Vanced Music APK Latest Version for Android You can even access your private music library from your device and play them through the app. The app also gives users full control over their streaming settings, such as limiting mobile data usage or preventing music videos from playing, allowing them to save a lot of their data. This mod was created by the same Vanced team that came up with YouTube Vanced and aims to add a few extra features such as background playbacks and in-app adblocking. And you can search and play the videos on Tube.Ģ.Vanced Music APK is a modded version of YouTube Music Premium that offers users the full premium experience of YouTube Music for free. All the free video is provided by Tube services. Vanced Tube using the third-party API source from Tube. ![]() Currently this app is only available for android but it gained a lot of popularity just with android users.Īs stated on the Vanced Kit, Vanced Kit is “a well known modded version of tube Video with many features such as adblocking and background playback and many more.ġ. You have many good things which can enhance you to use this app. Mainly you can fell like using a normal tube app but a premium version of it. There are many things that made this app so popular. With You Vanced Tube Videos - Play tube: you can search for your favorite videos, create your own playlists, discover new videos, and browse the daily Top Trend of your country, unlimited video watching! It has a top popup player you can watch videos while doing other activities. You Vanced Tube Videos - Block All Ads for you and vanced will help you watch millions of music, documentary and videos. Currently this app is only available for android but it gained a lot of popularity just with android users. You can install it on google play.Ī: As stated on the Vanced Kit, Vanced Kit is “a well known modded version of tube Video with many features such as adblocking and background playback and many more.Ī:There are many things that made this app so popular. Which Video Tube Vanced should I choose: Root or Non-root?Ī: We provide a completely Non-root version of Vanced Kit. How to install Vanced Kit Non-root variant without Video Tube Vanced Manager?Ī: Find Vanced Kit - Block All Ads For Tube Vanced and install it. How to use Vanced Kit - Block All Ads For You Vanced?Ī: Just like opening tube app, open the app to browse videos or search for videos, You will find that the video is completely ad-free, All ads have been removed. Discover free music with tons of predefined genres: Popular Music, Hip Hop, Rock, Classical, R&B, Jazz, Disco, Country and more Maximum resolution of videos active by default. No need to install other plug-ins, such as microG, Vanced Manager Supports multiple languages: Spanish, Russian, Portuguese, English, Indonesian, Japanese, Korean, Thai, etc.Įnjoy Unlimited Vanced Tube videos ,remove ADs! More convenient installation, NO ADS, no need for microG. Vanced App - Block All Ads for you and vanced music, documentary and videos. ![]() ![]() ![]() ![]() In total, approximately 10,000 regions of the genome were determined as differentially accessible regions (DARs) upon induction of bZIP11 translocation to the nucleus, depending on which control was used (Table 1). Approximately 50,000 cells from each replicate of each treatment were used for ATAC-seq. In parallel, the samples were treated, prior to DEX or acetone treatment, with cycloheximide (CHX), a protein biosynthesis inhibitor 21 that enables to discriminate the direct targets of bZIP11 from its indirect targets. Using protoplasts allows for a rapid and homogeneous chemical uptake which reduces variability between samples 20. Four biological replicates were treated with either DEX, which induces the translocation of bZIP11 protein into the nucleus, or acetone, the solvent for DEX (Mock control). Protoplasts were treated for 45 minutes after a one hr recovery period following protoplast extraction. ![]() Four-week-old Arabidopsis plants were used to extract leaf protoplasts from approximately six leaves per plant (experiment summarised in Fig. ![]() Upon treatment with dexamethasone (DEX), which binds to the HBD, the translocation of bZIP11 to the nucleus is enabled, allowing its TF activity to occur 19. A rat glucocorticoid receptor, or hormone binding domain (HBD), is fused to bZIP11, and retains this TF in the cytoplasm. In these p35S:bZIP11-HBD plants, the expression of bZIP11 coding sequence is driven by the cauliflower mosaic virus, CaMV35S, promoter ( p35S) that leads to ectopic gene expression in the whole plant. To achieve a comprehensive profile of accessible chromatin regions and discover new downstream targets of bZIP11, we generated 24 chromatin accessibility data sets from wild-type (WT) Arabidopsis protoplast samples, as well as from transgenic p35S:bZIP11-HBD Arabidopsis plants 18. Here we report ATAC-seq results in response to induction of a single plant TF, with known regulatory sequences, to determine the genome-wide effect of this TF on chromatin accessibility in Arabidopsis. Previous studies have performed ATAC-seq on different plant organs, conditions, or in response to different treatments to determine changes in accessibility of those DNA regions that are likely to contain regulatory sequences 14, 15, 16, 17. This technique is rapid and can be performed on small quantities of starting material. However, the extent to which bZIP11 impacts chromatin accessibility at the whole-genome scale remains unknown.Īssay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) is a technique which can determine genome-wide locations of chromatin accessible to transposase insertion and by extension, other proteins, for example TFs 12, 13. bZIP11 has been demonstrated to promote the expression of specific auxin-related genes by recruiting histone acetylation machinery to enhance chromatin accessibility 3, 11. The bZIP TFs bind DNA at cis-regulatory elements (CREs) known as G-boxes, which all have a conserved ACGT core flanked by cytosines or guanines (C/GACGTG/C) 10. It is estimated by DNA Affinity Purification with high throughput sequencing (DAP-seq) that bZIP11 contains DNA-binding sites in over 7,000 genes in Arabidopsis, which is nearly one third of the entire genes in the genome 9. In Arabidopsis thaliana (Arabidopsis), bZIP11 belongs to a group of five proteins involved in sugar responses, named the S 1 bZIP group 2, 4, 5, 6, 7, 8. Our study provides a resource for understanding how bZIP11 regulates the genome at the chromatin level and provides an example of the impact of a single transcription factor on a whole plant genome.īasic leucine zipper11 (bZIP11) is a transcription factor (TF) which regulates gene expression during low-energy conditions in plants and enables plants to adjust their metabolism, growth, and development to such unfavourable conditions 1, 2, 3. We identified, on average, 10,000 differentially accessible regions upon bZIP11 induction, corresponding to over 8,420 different genes out of the 25,000 genes present in the Arabidopsis genome. Here we addressed this by performing an ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) on Arabidopsis thaliana (Arabidopsis) leaf protoplasts to obtain a profile of chromatin patterning in response upon bZIP11 induction. Although previous results indicate that bZIP11 regulates chromatin accessibility based on evidence obtained from single genomic loci, to what extent this transcription factor regulates the chromatin landscape at the whole genome level remains unknown. Basic leucine zipper 11 (bZIP11) is a transcription factor that is activated under low energy conditions in plants and plays a crucial role in enabling plants to adapt to starvation situations. ![]() ![]() ![]() Set the program to clear the recorded results after every five measurements. Select the color theme, activate the Stackmat timer or change the font.Ĭhoose to hide certain interface panels while the timer is running or hide the timer itself and reveal the result only when the timer has stopped. Customizing the DesignĬustomize the look of the timer choosing from the available designs. Turn on the inpection time to delay the timer with 3, 10 or 15 seconds. These general scramble options wary depending on the selected puzzle. Customize scramble options setting a custom length (number of rotations), initial orientation, separate the string for a better readability or set it to use the F R U rotations only. Select the puzzle you're practicing with and the puzzle scrambler will generate a random scramble according to this setting. SettingsĬlick the gear icon in the upper corner of the screen to access the settings panel. ![]() Add +2 or DNF penalties, according to official WCA regulations if necessary. Release the key and press it again to stop the counter when you finished the solution. Press the space button on your keyboard or click/touch the main timer until the green light indicates that the timer is ready to start. The Cube Timer adjusts itself to the current screen size, supports keyboard, mouse and touchscreens working great both on desktop and mobile devices. Export your preferred settings and save them in a custom link. Save your times and statistics to the cloud or in a text file. Inspection time, customizable design and various other options help you enjoy your puzzle practice. The stopwatch collects and analizes training statistics, calculates averages for 5 and 12 times and shows the evolution of performance on a graphical display. Online Rubik's Cube speedsolving timer with built-in scramble generator for WCA twisty puzzles. ![]() |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |